Coding

Part:BBa_K5142043:Design

Designed by: Yuchen Liang   Group: iGEM24_HunanU   (2024-10-01)


Vaccinia virus A26L (partial)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 523
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 524
    Illegal PstI site found at 538
    Illegal NotI site found at 7
    Illegal NotI site found at 531
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 524
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 524
    Illegal PstI site found at 538
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our project, the A26L DNA sequence was served as a homologous arm for recombination only. Thus, the A26L sequence was not necessarily to be complete cds. Considering the whole A26L sequence is too long as a homologous arm, we chose 500 bp sequence adjacent to A27L only to construct the recombinant element.


Source

The partial sequence of A26L was cloned by PCR amplification from the genome of vaccinia virus (GenBank ID: JX489136.1).

References